Lens culinaris

Overview
GenusLens
Speciesculinaris
Common NameCultivated Lentil
AbbreviationL.culinaris

Lentil (Lens culinaris Medik.) is an important pulse crop with annual production of 3-4 Mt across 70 countries (Cubero et al. 2009. DOI 10.1079/9781845934873.0000; pg. 13). Lentils are a good source of protein, carbohydrates, micronutrients and vitamins for human nutrition and is consumed in more than 120 countries. Furthermore, their small seed size and flat shape make them relatively quick cooking and easily decorticated compared to most other grain legumes (Sharpe et al. 2013. BMC Genomics. DOI 10.1186/1471-2164-14-192). The Lentil plant has a bushy growth habit with a height of about 40 cm; the seeds are lens-shaped and usually grow two per pod.

Breeding at the University of Saskatchewan

Market Classes: Large Green, Medium Green, Small Green, Small Red, Extra Small Red, as well as, a Few Minor Specialty Classes.

Breeding Objectives: High Yield, Lodging Tolerance, Appropriate Size, Shape, Seed Coat Colour & Quality, and Resistance to Ascochyta Blight, Anthracnose, Stemphylium Blight & Botrytis .

Germplasm Data
The following germplasm data is currently available:
Stock TypeCount
Single Cross2,321
Single Cross2,321
Triple Cross1,498
Triple Cross1,498
Individual1,025
Individual1,025
DNA472
DNA472
Multiple Cross365
Multiple Cross365
variety358
variety358
Backcross243
Backcross243
Double Cross126
Double Cross126
Population58
Population58
Sequence & Variant Data
The following sequence and variant data are currently present:
Feature TypeCount
marker56,562
marker56,562
SNP52,183
SNP52,183
contig28,939
contig28,939
EST9,513
EST9,513
MNP1,543
MNP1,543
read_pair1,206
read_pair1,206
indel789
indel789
Nutritional Facts

Lentils, raw (dry weight)

Energy
343.00
kcal
Carbohydrate, by difference
60.08
g
Fiber, total dietary
30.50
g
Sugars, total
2.03
g
Projects
2014
A number of KASP markers were developed based on the genotypes identified under the Lentil 454 Sequencing Project. An initial set were used for validation of the SNP calling before developing the Illumina Golden Gate Assay (Lc1536). An additional 350 KASP primers were then designed for the SNPs that were successfully mapped using data from the GoldenGate array (see Fedoruk et al. 2013).
2012
Lentil seed is a good source of phenolic compounds, which can have health benefits. This project will try to find how different seed coat colours in lentil can be related to the phenolics profile. A fast extraction method and an optimized LC-MS analysis were applied to compare green, gray, tan, and brown seed coat colour lentils. Also, the so called zero-tannin genotypes were compared with the normal ones based upon their phenolic profile. The effect of storage on phenolic profile of lentil seeds was investigated, as well.
2010 to 2012
The nutritional value of pea, lentil, chickpea and dry bean grains are highly important for human health. Biofortification, enriching the nutritional contribution of staple crops through plant breeding, is one option that is now widely discussed in the fields of nutrition and public health at the national and international levels.
2006 to 2011
In this research, we plan to investigate how lentil indeterminacy can be managed by four strategies. The first is to test if lentil maturity can be controlled by soil N supply in zero tillage and conventional tillage soils. The second is to test if a desiccant at low concentrations can trigger senescence and maturity under high N conditions. The third strategy is to test if nine lentil genotypes that are commercially grown vary in N uptake, N fixation, or N redistribution within the plant during reproductive growth. Finally, the fourth strategy is to identify earlier establishing and earlier senescing rhizobia strains so N fixation will only occur up to mid-reproductive growth. Together, all these strategies will give us a detailed understanding of how N is partitioned in lentil in order to have satisfactory crop maturity.
2011
An Illumina Golden Gate array was developed using SNPs identified as part of the Lentil 454 Sequencing & Genotyping Project. Loci where chosen such that the SNPs should be distributed evenly across the genome based on comparison to Medicago truncatula.
2009
Lentil is an economically important pulse crop for Canada produced mainly for the export market. In conventional breeding programs, several segregating generations must be grown in order to reach a certain level of homozygosity that allows the selection of traits of interest. In contrast, double-haploid (DH) technology produces instant homozygosity and thus can significantly reduce the time required for developing new varieties. The efficiency of the lentil breeding program will also be improved through the reduction in the population size required for screening.
2009
Colletotrichum truncatum is a pathogen of several leguminous plant species where it causes a disease called anthracnose. Host specialization, infertility and genetic differentiation among isolates from different hosts have been demonstrated. In the population of C. truncatum from lentil two races were described. Detailed histological studies of the infection process by isolates of both races have revealed quantitative rather than qualitative differences.
2009
Lentil anthracnose is currently the most important lentil disease in Saskatchewan. In a project about to be completed on molecular aspects of this pathogen, we generated an extensive library of genes activated by the anthracnose fungus and by lentil during the infection process. In order to fully benefit from the investment made in this NSERC-CRD, we received one-year funding to confirm the function of a number of genes in the anthracnose fungus, Colletotrichum truncatum, that we have identified and believe to be involved in virulence of the fungus.
2009
Ninety-six Lentil Association mapping panel (LAM) lines were run on the Lc1536 Lentil Illumina Golden Gate assay.
2009
Ninety-six Lentil Association mapping panel (LAM) lines were run on the Lc1536 Lentil Illumina Golden Gate assay.

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