Lens ervoides is a wild relative of Lens culinaris with semi-hastate or lanceolate stipules. L. ervoides can be distinguished from other wild Lentil species by its smaller leaves, calyx teath, pods and seeds1. L. ervoides is often found in shady or partially shady niches, such as among bushes or under trees, with stony soils1. Unlike other wild lentil species, L. ervoides is rarely found in mixed stands with other wild lentils1. Recent sequence analysis indicates that Lens ervoides is in the tertiary gene pool of L. culinaris2
The following germplasm data is currently available:
2014 to 2017
Using Crop Wild Relatives for Future Lentil Breeding: Evaluation of Drought and Disease Resistance of Interspecific Hybrid Lines
This is an international project funded by the Global Crop Diversity Trust aimed at evaluating cultivated x wild lentil introgression lines for multiple traits in multiple environments.
2013 to 2016
Lentils are seen as a source for essential vitamins and minerals for human nutrition, but due to the high anti-nutritional factors of raffinose family oligosaccharides the consumption of lentils are being limited. Other methods to lower the levels of these RFOs are costly, and that is why an alternative strategy to develop varieties of lentil with lower levels is being implemented.
A number of KASP markers were developed based on the genotypes identified under the Lentil 454 Sequencing Project. An initial set were used for validation of the SNP calling before developing the Illumina Golden Gate Assay (Lc1536). An additional 350 KASP primers were then designed for the SNPs that were successfully mapped using data from the GoldenGate array (see Fedoruk et al. 2013).
Lentil seed is a good source of phenolic compounds, which can have health benefits. This project will try to find how different seed coat colours in lentil can be related to the phenolics profile. A fast extraction method and an optimized LC-MS analysis were applied to compare green, gray, tan, and brown seed coat colour lentils. Also, the so called zero-tannin genotypes were compared with the normal ones based upon their phenolic profile. The effect of storage on phenolic profile of lentil seeds was investigated, as well.
A set of 1107 legume cross species orthologous sequences (COS) were amplified from Lens culinaris (CDC Redberry and Eston) and L. ervoides (L01-827a and IG 72815). Sequences were aligned and SNPs identified. A subset of 110 KASP assays were designed for use in L. culinaris. An Illumina GoldenGate array of 768 SNPs was designed for use in L. ervoides or interspecies hybrid populations between Lc and Le.
Lentil has been grown commercially in western Canada since 1970. Ascochyta lentis, the causal agent of ascochyta blight of lentil is established as one of the most economically important diseases of lentil in Western Canada. To deal with this problem, the widely acceptable genetic improvement strategy is to pyramid resistance genes. Developing closely linked single nucleotide polymorphism (SNP) markers for resistance genes is prerequisite for pyramiding resistance genes. To develop SNP markers, a series of selected recombinant inbred line (RIL) populations derived from resistant sources will be phenotyped under greenhouse conditions (pathogenicity tests) followed by screening available SNP markers across the entire set of RIL populations.