AGILE: Application of Genomic Innovation in the Lentil Economy

2015 to 2019

AGILE Project Logo

Development of improved lentil cultivars well-adapted to the local environment is an on-going process in the breeding program and is critical for long-term genetic gain. Recent climate instability adds another layer of complexity to breeding efforts. Continued genetic improvement of lentil will, therefore, involve the introduction of new alleles that extend beyond the existing adapted pool of germplasm. Our goal in AGILE is to enhance the productivity and quality of Canadian lentils by expediting the expansion of genetic diversity of the Canadian lentil germplasm base with the use of genomic technologies.

In this project, we will first characterize the genetic variability available within the primary and secondary gene pools of the genus Lens through extensive genotyping and phenotyping. The information will be used to determine the genetic basis of the contrasting adaptation characteristics of lentils from the three main growing regions: northern temperate, mediterranean and S. Asia. We will then develop breeder-friendly markers for tracking response to photoperiod, temperature and light quality, and generate resources and tools to allow breeders to better use exotic germplasm and wild relatives while reducing any negative impacts. A systematic study of symbiont diversity will allow for a better understanding of ways to improve the nitrogen fixation process in lentils.

Access to superior cultivars does not automatically translate to adoption by farmers, particularly if they are unfamiliar with lentil crop production. Numerous factors influence crop production decisions, and a clearer understanding of these will help increase producer uptake of this important crop. Our GE3LS research will identify the various factors that may influence producer decision making processes and propose a strategy for effective communication and knowledge exchange/transfer, which will encourage sustainable and profitable production of lentils in Canada.

Results of AGILE will allow us to develop a thorough understanding of lentil and its wild relatives. Resources developed by this project will improve the agility of the lentil breeding program by introducing genetic diversity with greater precision, and speed up the breeding cycle. This project will also foster international partnership, which is critical for long-term success of the international lentil community.

Additional information about this project:
Property NameValue
TypeResearch Experiment
Research Area
Breeding & Genetics

Plant breeding is the art and science of changing the traits of plants in order to produce desired characteristics. Plant breeding can be accomplished through many different techniques ranging from simply selecting plants with desirable characteristics for propagation, to more complex molecular techniques. ... [more]

Related Species

Lentil (Lens culinaris Medik.) is an important pulse crop with annual production of 3-4 Mt across 70 countries (Cubero et al. 2009. DOI 10.1079/9781845934873.0000; pg. 13). Lentils are a good source of protein, carbohydrates, micronutrients and vitamins for human nutrition and is consumed in more than 120 countries. Furthermore, their small seed size and flat shape make them relatively quick cooking and easily decorticated compared to most other grain legumes (Sharpe et al. 2013. BMC Genomics. DOI 10.1186/1471-2164-14-192). The Lentil plant has a bushy growth habit with a height of about 40 cm; the seeds are lens-shaped and usually grow two per pod. ... [more]


Lens lamottei is a wild relative of Lens culinaris with horizontal, less dentate stipules1. Recent sequence analysis indicates that Lens lamottei is in the secondary gene pool of L. culinaris2. ... [more]


Lens odemensis is a wild relative of Lens culinaris with semi-hastate stipules which form horizontal positions on the stem1. Recent sequence analysis indicates that Lens odemensis is in the secondary gene pool of L. culinaris2. ... [more]


Lens orientalis is a wild relative of Lens culinaris with lanceolate stipules. The geographical distribution of Lens orientalis ranges from Turkey to Uzbekistan with a primary habitat of stony and gravelly niches where aggressive annuals are not successful1. Lens orientalis usually forms small disjunct populations containing a small number of plants in sparse stands1. More extensive populations of L. orientalis were found at high elevations (800 to 2,000 m)1. Recent sequence analysis indicates that Lens orientalis is in the primary gene pool of L. culinaris2 ... [more]


Lens tomentosus is a wild relative of Lens culinaris. Morphologically, L. tomentosus most resembles L. orientalis although it can be distinguished as having a hairy pod2. Recent sequence analysis indicates that Lens orientalis is in the primary gene pool of L. culinaris2. ... [more]

There are 324 Accessions used in this project.Download List
Namesort ascendingOriginSpeciesType
ILL 6853SyriaLens culinarisAccession
ILL 7089RussiaLens culinarisAccession
ILL 7558IndiaLens culinarisAccession
ILL 7663SyriaLens culinarisAccession
ILL 7668SyriaLens culinarisAccession
ILL 7716IndiaLens culinarisAccession
ILL 7747SyriaLens culinarisAccession
ILL 7946SyriaLens culinarisAccession
ILL 7978EthiopiaLens culinarisAccession
ILL 7979SyriaLens culinarisAccession
ILL 8007BangladeshLens culinarisAccession
ILL 8009BangladeshLens culinarisAccession
ILL 8010BangladeshLens culinarisAccession
ILL 8072IndiaLens culinarisAccession
ILL 8174NepalLens culinarisAccession
ILL 8595EcuadorLens culinarisAccession
ILL 9JordanLens culinarisAccession
ILL 975ChileLens culinarisAccession
ILL 9888SyriaLens culinarisAccession
ILL 9932SyriaLens culinarisAccession
ILL 9945SyriaLens culinarisAccession
ILL 9977SyriaLens culinarisAccession
ILL 9997SyriaLens culinarisAccession
ILWL 118SyriaLens orientalisAccession
IndianheadCanadaLens culinarisVariety
IPL 220IndiaLens culinarisAccession
LairdCanadaLens culinarisVariety
LIRL-22-46IndiaLens culinarisAccession
PI 163589GuatemalaLens culinarisAccession
PI 169534 LSPTurkeyLens culinarisAccession
PI 175754 LSPTurkeyLens culinarisAccession
PI 177430 LSPTurkeyLens culinarisAccession
PI 178939 LSPTurkeyLens culinarisAccession
PI 178952TurkeyLens culinarisAccession
PI 178971 LSPTurkeyLens culinarisAccession
PI 179324 LSPSyriaLens culinarisAccession
PI 179330TurkeyLens culinarisAccession
PI 181771 LSPLebanonLens culinarisAccession
PI 181886 LSPSyriaLens culinarisAccession
PI 182217 LSPTurkeyLens culinarisAccession
PI 193546EthiopiaLens culinarisAccession
PI 193547 LSPEthiopiaLens culinarisAccession
PI 193548 LSPEthiopiaLens culinarisAccession
PI 193550EthiopiaLens culinarisAccession
PI 207492 LSPAfghanistanLens culinarisAccession
PI 209447Costa RicaLens culinarisAccession
PI 209858 LSPGreeceLens culinarisAccession
PI 211052 LSPAfghanistanLens culinarisAccession
PI 212100 LSPAfghanistanLens culinarisAccession
PI 212610 LSPAfghanistanLens culinarisAccession


Sequences, Variants & Markers