AGILE: Application of Genomic Innovation in the Lentil Economy

Overview
2015 to 2019

AGILE Project Logo

Development of improved lentil cultivars well-adapted to the local environment is an on-going process in the breeding program and is critical for long-term genetic gain. Recent climate instability adds another layer of complexity to breeding efforts. Continued genetic improvement of lentil will, therefore, involve the introduction of new alleles that extend beyond the existing adapted pool of germplasm. Our goal in AGILE is to enhance the productivity and quality of Canadian lentils by expediting the expansion of genetic diversity of the Canadian lentil germplasm base with the use of genomic technologies.

In this project, we will first characterize the genetic variability available within the primary and secondary gene pools of the genus Lens through extensive genotyping and phenotyping. The information will be used to determine the genetic basis of the contrasting adaptation characteristics of lentils from the three main growing regions: northern temperate, mediterranean and S. Asia. We will then develop breeder-friendly markers for tracking response to photoperiod, temperature and light quality, and generate resources and tools to allow breeders to better use exotic germplasm and wild relatives while reducing any negative impacts. A systematic study of symbiont diversity will allow for a better understanding of ways to improve the nitrogen fixation process in lentils.

Access to superior cultivars does not automatically translate to adoption by farmers, particularly if they are unfamiliar with lentil crop production. Numerous factors influence crop production decisions, and a clearer understanding of these will help increase producer uptake of this important crop. Our GE3LS research will identify the various factors that may influence producer decision making processes and propose a strategy for effective communication and knowledge exchange/transfer, which will encourage sustainable and profitable production of lentils in Canada.

Results of AGILE will allow us to develop a thorough understanding of lentil and its wild relatives. Resources developed by this project will improve the agility of the lentil breeding program by introducing genetic diversity with greater precision, and speed up the breeding cycle. This project will also foster international partnership, which is critical for long-term success of the international lentil community.

Properties
Additional information about this project:
Property NameValue
TypeResearch Experiment
Research Area
Breeding & Genetics

Plant breeding is the art and science of changing the traits of plants in order to produce desired characteristics. Plant breeding can be accomplished through many different techniques ranging from simply selecting plants with desirable characteristics for propagation, to more complex molecular techniques. ... [more]

 
Related Species

Lentil (Lens culinaris Medik.) is an important pulse crop with annual production of 3-4 Mt across 70 countries (Cubero et al. 2009. DOI 10.1079/9781845934873.0000; pg. 13). Lentils are a good source of protein, carbohydrates, micronutrients and vitamins for human nutrition and is consumed in more than 120 countries. Furthermore, their small seed size and flat shape make them relatively quick cooking and easily decorticated compared to most other grain legumes (Sharpe et al. 2013. BMC Genomics. DOI 10.1186/1471-2164-14-192). The Lentil plant has a bushy growth habit with a height of about 40 cm; the seeds are lens-shaped and usually grow two per pod. ... [more]

 

Lens lamottei is a wild relative of Lens culinaris with horizontal, less dentate stipules1. Recent sequence analysis indicates that Lens lamottei is in the secondary gene pool of L. culinaris2. ... [more]

 

Lens odemensis is a wild relative of Lens culinaris with semi-hastate stipules which form horizontal positions on the stem1. Recent sequence analysis indicates that Lens odemensis is in the secondary gene pool of L. culinaris2. ... [more]

 

Lens orientalis is a wild relative of Lens culinaris with lanceolate stipules. The geographical distribution of Lens orientalis ranges from Turkey to Uzbekistan with a primary habitat of stony and gravelly niches where aggressive annuals are not successful1. Lens orientalis usually forms small disjunct populations containing a small number of plants in sparse stands1. More extensive populations of L. orientalis were found at high elevations (800 to 2,000 m)1. Recent sequence analysis indicates that Lens orientalis is in the primary gene pool of L. culinaris2 ... [more]

 

Lens tomentosus is a wild relative of Lens culinaris. Morphologically, L. tomentosus most resembles L. orientalis although it can be distinguished as having a hairy pod2. Recent sequence analysis indicates that Lens orientalis is in the primary gene pool of L. culinaris2. ... [more]

 
Germplasm
There are 324 Accessions used in this project.Download List
Namesort ascendingOriginSpeciesType
ILL 6821 AGLLens culinarisAccession
ILL 6853 AGLLens culinarisAccession
ILL 7089 AGLLens culinarisAccession
ILL 7558 AGLLens culinarisAccession
ILL 7663 AGLLens culinarisAccession
ILL 7668 AGLLens culinarisAccession
ILL 7716 AGLLens culinarisAccession
ILL 7747 AGLLens culinarisAccession
ILL 7946 AGLLens culinarisAccession
ILL 7978 AGLLens culinarisAccession
ILL 7979 AGLLens culinarisAccession
ILL 8007 AGLLens culinarisAccession
ILL 8009 AGLLens culinarisAccession
ILL 8010 AGLLens culinarisAccession
ILL 8072 AGLLens culinarisAccession
ILL 8174 AGLLens culinarisAccession
ILL 8595 AGLLens culinarisAccession
ILL 975 AGLLens culinarisAccession
ILL 9888 AGLLens culinarisAccession
ILL 9932 AGLLens culinarisAccession
ILL 9945 AGLLens culinarisAccession
ILL 9977 AGLLens culinarisAccession
ILL 9997 AGLLens culinarisAccession
ILL 9 AGLLens culinarisAccession
ILWL 118 AGLLens orientalisAccession
Indianhead AGLLens culinarisAccession
IPL 220 AGLLens culinarisAccession
Laird AGLLens culinarisAccession
LIRL-22-46 AGLLens culinarisAccession
PI 163589 AGLLens culinarisAccession
PI 169534 LSP AGLLens culinarisAccession
PI 175754 LSP AGLLens culinarisAccession
PI 177430 LSP AGLLens culinarisAccession
PI 178939 LSP AGLLens culinarisAccession
PI 178952 AGLLens culinarisAccession
PI 178971 LSP AGLLens culinarisAccession
PI 179324 LSP AGLLens culinarisAccession
PI 179330 AGLLens culinarisAccession
PI 181771 LSP AGLLens culinarisAccession
PI 181886 LSP AGLLens culinarisAccession
PI 182217 LSP AGLLens culinarisAccession
PI 193546 AGLLens culinarisAccession
PI 193547 LSP AGLLens culinarisAccession
PI 193548 LSP AGLLens culinarisAccession
PI 193550 AGLLens culinarisAccession
PI 207492 LSP AGLLens culinarisAccession
PI 209858 LSP AGLLens culinarisAccession
PI 211052 LSP AGLLens culinarisAccession
PI 212100 LSP AGLLens culinarisAccession
PI 212610 LSP AGLLens culinarisAccession

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Sequences, Variants & Markers