Iron bioavailability in low phytate pea

Overview
2013 to 2015

The objectives of this study are to determine the effect of genotype and environment on iron bioavailability in a set of five pea varieties differing in phytate concentration using the Caco-2 mammalian cell bioassay, to determine whether iron bioavailability in field pea is heritable by evaluating recombinant inbred lines differing in phytate concentration using the Caco-2 mammalian cell bioassay, and to determine the effect of the pea low phytate trait on chicken performance and iron bioavailability in chicken.

In a previous study, two low phytate pea lines (1-2347-144 and 1-150-81) were developed from CDC Bronco at the Crop Development Centre of University of Saskatchewan. As a powerful chelator of iron, phytate can reduce the iron bioavailability in diets.  The low phytate peas may have increased iron bioavailability compared to the normal phytate peas.  In this project, the comparison of iron bioavailability among the two low phytate pea lines, their parent CDC Bronco and two other popular pea varieties in western Canada (CDC Meadow and CDC Golden), which are derived from 3 replicate field experiments conducted by M.Sc. student Tamira Delgerjav in 2009 and 2010, will be conducted using the methods of Caco-2 mammalian cell bioassay. The seeds from these varieties will be increased at Meath Park, Saskatchewan in 2011 and 2012 for the in vivo chicken study.  The low phytate line 1-2347-144 and CDC Meadow were crossed to develop F7 recombinant inbred lines (RILs) from the experiment of Mr. Arun Shunmugam. The inheritance of iron bioavailability will be tested by evaluating F7 RILs and their parents using in vitro Caco-2 mammalian cell bioassay.

Properties
Additional information about this project:
Property NameValue
TypeResearch Experiment
Research Area
Breeding & Genetics

Plant breeding is the art and science of changing the traits of plants in order to produce desired characteristics. Plant breeding can be accomplished through many different techniques ranging from simply selecting plants with desirable characteristics for propagation, to more complex molecular techniques. ... [more]

 
Physiology

The objectives of the Pulse Research Group Physiology Program is to investigate whole plant and field responses of crops, particularly pulse crops, to nutrient, water and weather.  To understand and improve yield formation in pulse crops in a warming climate.  To investigate and improve nitrogen ... [more]

 
Related Species

Pea (Pisum sativum L.) is one of the first domesticated crops, and was the model crop for the foundational genetic studies by Gregor Mendel, which he first reported in 1865. Pea is grown in most temperate regions of the world with annual production over the past decade of 10-12 million tonnes of field pea and 14-17 million tonnes of vegetable pea. Pea belongs to the Leguminosae family and consists of two species, P. fulvum and P. sativum with several ‘wild’ subspecies of P. sativum. Canada is the leading producer and exporter of field pea in the world. Saskatchewan is the leading province in pea production followed by Alberta and Manitoba. ... [more]

 
Sequences, Variants & Markers