The genetics of raffinose family oligosaccharides content and introgression in seeds
2013 to 2016
The lentil is a good source of nutrients, vitamins, and minerals for human consumption. Through the increasing demand of nutrition sources for populations in developing nations, the carbohydrate composition of lentil has been recognized as a good nutritional food source. However, along with the amount of soluble carbohydrate found in the lentil, also present are sucrose and raffinose family oligosaccharides (RFOs) which is indigestible for humans due to the lack of ɑ-galactosidase to break down the linkage of side chain. Because of this, RFOs are frequently regarded as being anti-nutritional and, due to the high content of RFOs in pulse crops, people will limit their consumption of these crops. This makes reducing RFO levels in crop species to be an important breeding goal.
Different methods of removing or reducing the levels of RFOs in lentils – such as: soaking, cooking, germinating, or fermenting the seeds prior to consumption – can be either expensive or time consuming, which would then increase production costs and decrease the use of lentils in the human diet. An alternate strategy would be to attempt at developing varieties of lentil with lower RFOs through breeding with some of the wild lentil relatives. The lentil wild relative L. ervoides was found to have nearly half the RFO concentration of the cultivated varietiesand may be an ideal genetic resource for genetic study of RFOs content in lentil.
The purposes of this study are to identify the genomic loci controlling the trait of lower RFO levels from wild relative donors, and to establish the genetic approach of fixing this trait from wild lentil species for breeding. This study will include an identification of the introgression from L. ervoides to L. culinaris, and will map the QTLs associated with seed RFOs content using two L.e. * L.c. RIL populations, while also estimating the QTL and environmental interaction on the RFOs in the seeds.
Sequences, Variants & Markers