Development of a fast extraction method and optimization of liquid chromatography-mass spectrometry for the analysis of phenolic compounds in lentil seed coats

Overview
TitleDevelopment of a fast extraction method and optimization of liquid chromatography-mass spectrometry for the analysis of phenolic compounds in lentil seed coats
AuthorsMirali Mahla, Ambrose Stephen J, Wood Stephen A, Vandenberg Albert, Purves Randy W
TypeJournal Article
JournalJournal of chromatography. B, Analytical technologies in the biomedical and life sciences
Volume969
CopyrightCopyright © 2014 Elsevier B.V. All rights reserved.
DOI10.1016/j.jchromb.2014.08.007
eISSN1873-376X
ElocationS1570-0232(14)00516-9
ISSN1873-376X
Journal AbbreviationJ. Chromatogr. B Analyt. Technol. Biomed. Life Sci.
Journal CountryNetherlands
LanguageEnglish
Language Abbreng
PIIS1570-0232(14)00516-9
Publication Date2014 Oct 15
Publication ModelPrint-Electronic

Abstract

<p>A systematic set of optimization experiments was conducted to design an efficient extraction and analysis protocol for screening six different sub-classes of phenolic compounds in the seed coat of various lentil (Lens culinaris Medik.) genotypes. Different compounds from anthocyanidins, flavan-3-ols, proanthocyanidins, flavanones, flavones, and flavonols sub-classes were first optimized for use as standards for liquid chromatography mass spectrometry (LC-MS) with UV detection. The effect of maceration duration, reconstitution solvent, and extraction solvent were investigated using lentil genotype CDC Maxim. Chromatographic conditions were optimized by examining column separation efficiencies, organic composition, and solvent gradient. The results showed that a 1h maceration step was sufficient and that non-acidified solvents were more appropriate; a 70:30 acetone: water (v/v) solvent was ultimately selected. Using a Kinetex PFP column, the organic concentration, gradient, and flow rate were optimized to maximize the resolution of phenolic compounds in a short 30-min analysis time. The optimized method was applied to three lentil genotypes with different phenolic compound profiles to provide information of value to breeding programs.</p>

Citation

Mirali M, Ambrose SJ, Wood SA, Vandenberg A, Purves RW. Development of a fast extraction method and optimization of liquid chromatography-mass spectrometry for the analysis of phenolic compounds in lentil seed coats. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. 2014 Oct 15; 969:149-61.

Related Species
Related Species: 

Lentil (Lens culinaris Medik.) is an important pulse crop with annual production of 3-4 Mt across 70 countries (Cubero et al. 2009. DOI 10.1079/9781845934873.0000; pg. 13). Lentils are a good source of protein, carbohydrates, micronutrients and vitamins for human nutrition and is consumed in more than 120 countries. Furthermore, their small seed size and flat shape make them relatively quick cooking and easily decorticated compared to most other grain legumes (Sharpe et al. 2013. BMC Genomics. DOI 10.1186/1471-2164-14-192). The Lentil plant has a bushy growth habit with a height of about 40 cm; the seeds are lens-shaped and usually grow two per pod. ... [more]

 
Cross References
This publication is also available in the following databases:
DatabaseAccession
PMID: PubMedPMID:25173496
Research Area
Research Area: 
Physiology

The objectives of the Pulse Research Group Physiology Program is to investigate whole plant and field responses of crops, particularly pulse crops, to nutrient, water and weather.  To understand and improve yield formation in pulse crops in a warming climate.  To investigate and improve nitrogen ... [more]