Detection of common vetch (Vicia sativa L.) in Lentil (Lens culinaris L.) using unique chemical fingerprint markers

TitleDetection of common vetch (Vicia sativa L.) in Lentil (Lens culinaris L.) using unique chemical fingerprint markers
AuthorsThavarajah Pushparajah, Thavarajah Dil, Premakumara G A S, Vandenberg Albert
TypeEvaluation Studies
CopyrightCopyright © 2012 Elsevier Ltd. All rights reserved.
Journal AbbreviationFood Chem
Journal CountryEngland
Language Abbreng
Publication Date2012 Dec 15
Publication ModelPrint-Electronic


<p>Detection of adulteration of split red lentil (Lens culinaris L.) seeds with low level addition of split common vetch (Vicia sativa L.) is hampered by a lack of reliable detection methods. An analytical method was developed using high performance liquid chromatography with diode array detection (HPLC-DAD) based on two unique chemical markers found in common vetch: ß-cyanoalanine (BCA) and γ-glutamyl-ß-cyanoalanine (GCA). These two markers were present in samples of common vetch seed grown in Canada and Serbia. Authentic lentil samples grown in Canada, Australia, USA, Turkey, Syria, and Morocco had no detectable levels of these chemical markers. Commercial lentil samples for export from lentil processing plants in Saskatchewan, Canada, also had no detectable levels of GCA and BCA. The presence of vetch in intentionally adulterated lentil samples could be determined via chemical markers with a detection limit of 5% (w/w). The proposed method is a simple sample extraction and rapid HPLC analysis that could be widely used to detect intentional adulteration of lentils with common vetch.</p>


Thavarajah P, Thavarajah D, Premakumara GA, Vandenberg A. Detection of common vetch (Vicia sativa L.) in Lentil (Lens culinaris L.) using unique chemical fingerprint markers. Food chemistry. 2012 Dec 15; 135(4):2203-6.

Related Species
Related Species 

Lentil (Lens culinaris Medik.) is an important pulse crop with annual production of 3-4 Mt across 70 countries (Cubero et al. 2009. DOI 10.1079/9781845934873.0000; pg. 13). Lentils are a good source of protein, carbohydrates, micronutrients and vitamins for human nutrition and is consumed in more than 120 countries. Furthermore, their small seed size and flat shape make them relatively quick cooking and easily decorticated compared to most other grain legumes (Sharpe et al. 2013. BMC Genomics. DOI 10.1186/1471-2164-14-192). The Lentil plant has a bushy growth habit with a height of about 40 cm; the seeds are lens-shaped and usually grow two per pod. ... [more]

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PMID: PubMedPMID:22980791
Research Area
Research Area 

In the Pulse Crop Pathology Group we are interested in the biology of fungal and bacterial pathogens and their interaction with the legume host plants. The ultimate goal is to gain a better understanding of strategies employed by these pathogens to successfully invade and colonize pulse crops, and to explo ... [more]