2015 to 2017
Stone seeds, which are seeds that do not absorb water, are considered a negative seed quality characteristic because they need to be removed before commercial processing. A high physical dormancy at the end of seed development is found to be the cause of this issue, but it is not known how or when it develops. This project will focus on attempting to determine when the seeds begin to develop physical dormancy, and also how to avoid hard seededness through harvest times.
<p>For farmers, crops that quickly cover the ground soon after seeding ("ground-cover") generally mean fewer weeds and reduced need for in-crop herbicide applications. Plants that grow faster early in the growing season and larger as the season goes on also tend to produce higher seed yields. These traits, however, have generally been very difficult and time-consuming to quantify. Biomass, in particular, is rarely measured due to associated time, costs, and killing the entire plants early.</p><p>The goal of this project is to quantify ground-cover and plant volume using overhead imagery captured from unmanned aerial vehicles (UAV's) and handheld cameras. Image analysis is performed on 2-dimensional stitched images to determine ground-cover and on 3-dimensional point clouds to measure parameters of plant volume, which are then compared with actual above-ground biomass. These imaging can be done quickly and economically.&nbsp; No plant killing is needed so&nbsp; it is possible to collect data at multiple times throughout the growing season.</p><p>Ultimately, these imaging methods may be used to quickly and efficiently obtain plant growth and architecture information in breeding programs.</p>
An Illumina Golden Gate array was developed using SNPs identified as part of the Common Bean 454 Sequencing & Genotyping Project.
2014 to 2017
This is an international project funded by the Global Crop Diversity Trust aimed at evaluating cultivated x wild lentil introgression lines for multiple traits in multiple environments.
2013 to 2016
As production of the dry bean is moving towards short season growing regions such as Alberta and Saskatchewan, it is becoming increasingly important to find a way to develop abiotic stress tolerances for the dry bean. Through the incorporation of genes from other species, the stress tolerance capabilities of the dry bean will increase, making it less sensitive to its surrounding climate. The tepary bean was decided upon as the best genetic donor for improvement to the dry bean, and is now being evaluated in Saskatchewan and its international partners.
<p>&nbsp; &nbsp; We have many different types of lentil grown in over 50 countries around the world.&nbsp; The timing to grow the crop is different depending on where you are.&nbsp; In Canada, lentil is sown in May and harvested in August.&nbsp; Whereas in Nepal, lentil is sown in October and harvested in February the year after.&nbsp; In Mediterranean countries such as Italy, lentil is sown in October but won't be harvested until May/June.</p><p>&nbsp; &nbsp; Most lentil varieties only perform well under a specific climate and fail when they are grown in under a different climate.&nbsp; Yield is closely related to adaptation and that is why breeders tend to use only a few local varieties in their crosses.&nbsp; To allow breeders to expand their choices, we need to know how different lentils interact and adapt to different environments, i.e. changing daylength and temperature over the growing season.&nbsp; Better understanding of the genetic mechanism that affects how lentil grow and mature in a specific climatic condition will help breeders to more effectively choose the lines in their crosses.</p>
2013 to 2016
Our approach to sequencing the lentil genome is two-fold. First, we are generating a high quality draft genome for a single lentil genotype (CDC Redberry), including bulk sequencing, assembly of chromosomal ‘pseudomolecules’, and gene prediction and annotation. Secondly, we are re-sequencing various lentil accessions from around the globe to reveal the breath of genetic potential present in our germplasm resources. The outcome will give us i) an understanding of how modern breeding has re-shaped the lentil genome, ii) identification of genes and genomic interval that control agronomic traits, and iii) discovery of many genetic polymorphisms for future marker development, that together will add considerable resources to the breeder’s toolbox for lentil genetic improvement. More importantly, the results of this project will allow us to leverage knowledge of important trait based on conservation of genome-based features with other legume crops (such as Medicago and chickpea).
2013 to 2016
Lentils are seen as a source for essential vitamins and minerals for human nutrition, but due to the high anti-nutritional factors of raffinose family oligosaccharides the consumption of lentils are being limited. Other methods to lower the levels of these RFOs are costly, and that is why an alternative strategy to develop varieties of lentil with lower levels is being implemented.
2012 to 2015
This group is involved in a wide range of biotechnology projects that accelerate the legume breeding process. Double-haploid technology has been achieved in both chickpea and field pea by the CDC group in collaboration with colleagues in France and Australia. Efforts are underway to adapt this technology to lentil. Improving efficiency and integrating these techniques into routine breeding programs to enhance genetic gain are important long-term goals.
2013 to 2015
The objectives of this study are to determine the effect of genotype and environment on iron bioavailability in a set of five pea varieties differing in phytate concentration using the Caco-2 mammalian cell bioassay, to determine whether iron bioavailability in field pea is heritable by evaluating recombinant inbred lines differing in phytate concentration using the Caco-2 mammalian cell bioassay, and to determine the effect of the pea low phytate trait on chicken performance and iron bioavailability in chicken.